Do you test each new antibody you buy, or do you just trust the manufacturer’s validation data? I’ve been thinking about setting up a small internal control test for every new batch, but I’m not sure if it’s worth the effort.
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I completely agree about validating antibodies yourself. Even the best ones can behave differently depending on your setup. I now have a little folder of reference blots from previous tests — so I can compare signal intensity between lots. It’s also a great way to train new lab members. Once they see how much variation can happen, they stop assuming every antibody “just works.”